상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Pyrrolidine dithiocarbamate-induced macrophage inflammatory protein-2 gene expression is NF-κB-independent but c-Jun-dependent in macrophage cell line RAW 264.7

Authors 

W J SohnK W LeeY LeeJ H HanYong Kyung ChoeD S KimH J Kwon

Publisher 

Elsevier

Issue Date 

2005

Citation 

Molecular Immunology, vol. 42, no. 10, pp. 1165-1175

Keywords 

AP-1macrophagesMIP-2PDTCproinflammatory cytokinecytokineimmunoglobulin enhancer binding proteinmacrophage inflammatory protein 2mitogen activated protein kinaseprotein c jun

Abstract 

Pyrrolidine dithiocarbamate (PDTC) is a stable compound that acts as antioxidant or prooxidant, and is widely used to inhibit the activation of NF-κB. PDTC was also reported to activate NF-κB depending on its dose and metal ions in PC12 cells. In this work, we demonstrated a working mechanism of PDTC and its effects on the proinflammatory cytokine gene expression in a mouse macrophage cell line, RAW 264.7. PDTC alone induced NF-κB-independent MIP-2 promoter activation that can be assessed by transient transfection and confocal image analysis. The involvement of AP-1 transcription factor was noticed by promoter deletion/site-specific mutation analysis and electrophoretic mobility shift assay (EMSA). Among three different mitogen-activated protein kinase (MAPK) pathways tested, only the stress-activated protein kinase (SAPK)/Jun N-terminal kinase (JNK) pathway was significantly activated in RAW 264.7 cells after the stimulation with PDTC. Using pathway-specific inhibitors, we found that the SAPK/JNK pathway is clearly associated with PDTC-induced MIP-2 gene expression. Our experimental results indicate that PDTC-induced proinflammatory cytokine expressions are mediated by SAPK/JNK pathway, which activates AP-1.

ISSN 

0161-5890

Link 

http://dx.doi.org/10.1016/j.molimm.2004.11.016

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)