상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Molecular taxonomy of a soil actinomycete isolate, KCCM10454 showing neuroprotective activity by 16S rRNA and rpoB gene analysis

Authors 

B H LeeH KimH J KimY K LimK H ByunB HutchinsonChang-Jin KimY H KoK H LeeC Y ChaY H KookB J Kim

Publisher 

Microbiological Society of Korea

Issue Date 

2005

Citation 

Journal of Microbiology, vol. 43, no. 2, pp. 213-218

Keywords 

antiepileptic activityapoptosisepilepsyhippocampal cellrifampin resistant genotypestreptomyces3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromideanticonvulsive agentDNA directed RNA polymeraseglutamic acid

Abstract 

Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4,5-dimethylthiazol-2- yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampin-resistant genotype, Asn(AAC)442, according to the results of 16S rRNA and rpoB gene analyses.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)