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Title 

Antitumor activity of spinasterol isolated from Pueraria roots

 

Pueraria 뿌리에서 동정한 spinasterol의 항암효과

Authors 

Gook Che JeonM S ParkDo Young YoonC H ShinH S SinS J Um

Publisher 

Korean Society of Medical Biochemistry

Issue Date 

2005

Citation 

Experimental and Molecular Medicine, vol. 37, no. 2, pp. 111-120

Keywords 

breast neoplasmsestrogen replacementphytoestrogenspuerariareceptors, estrogenspinasteroltherapyalpha spinasterolpueraria extractpueraria lobata extract

Abstract 

We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria Iobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA-MB-231) in a dose- and time-dependent manner, as well as the growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17β-estradiol, which activates both estrogen receptor α (ERα) and ERβ. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root.

ISSN 

1226-3613

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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