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Title 

The first two-dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins

Authors 

N SunJ JangS LeeS KimKwang Lae HoeKyung Sook ChungDong Uk KimHyang Sook YooMi Sun WonK B Song

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

Proteomics, vol. 5, no. 6, pp. 1574-1579

Keywords 

matrix-assisted laser desorption/ionizationpeptide fingerprintingproteomeschizosaccharomyces pombetwo-dimensional gel electrophoresisheat shock proteinprotein analysisprotein determinationelectrophoresis, gel, two-dimensionalschizosaccharomyces

Abstract 

Cytosolic proteins of Schizosaccharomyces pombe were separated by two-dimensional (2-D) gel electrophoresis, to construct the first 2-D reference map. In the pI range 4-7, more than 500 spots were detected by silver staining, and 70 different proteins corresponding to 111 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and tandem mass spectrometry, where necessary. In the pI range 6-9, approximately 330 spots were detected, and 31 proteins corresponding to 38 spots were identified by mass spectrometry. More than 50% of the identified proteins were involved in amino acid, carbohydrate or nucleotide metabolism, and energy production. A second large group of identified proteins comprises heat shock and other stress related proteins and chaperones.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.200401053

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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