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Title 

BRD-glucan exhibits potent immunochemotherapeutic activity in vitro and in vivo

Authors 

Seong Hoon MoonJ C HeoR L FineHwan Mook KimSung Uk KimByung Dae YoonSang-Han Lee

Publisher 

Spandidos Publications

Issue Date 

2005

Citation 

International Journal of Oncology, vol. 26, no. 2, pp. 395-404

Keywords 

BRD glucan, aureobasidiumglucanglucans

Abstract 

We carried out in vitro and in vivo assays to investigate the immunomodulatory and immunochemotherapeutic action mechanism of BRD-glucan, a high molecular weight ( approximately 3,500 kDa) polysaccharide isolated from Aureobasidium sp, and assessed the efficacy of BRD-glucan/adriamycin co-treatment of animal cancer models. RT-PCR and suspension hemolytic, plaque forming, wounding, invasion and cell proliferation assays were utilized to investigate the in vitro immunochemotherapeutic effects of BRD-glucan. In vivo, the effects of BRD-glucan and BRD-glucan/adriamycin co-treatment were tested in a B16 melanoma initiation model and in C57BL/6 mice. In vitro, BRD-glucan did not affect the cellular wounding response or invasion activity; treatment with BRD-glucan led to increase proliferation of B cells, natural killer cells and macrophages, but not T cells. In addition, we found that the BRD-glucan activation of B cells and macrophages was dependent on Toll-like receptor2 (TLR2) and TLR4, which play important roles in innate and adaptive immunity. In vivo, BRD-glucan/adriamycin co-treatment effectively reduced the number and size of metastatic colonies. Based on the results of our in vitro and in vivo toxicity, safety and immunochemotherapy assays, we propose that BRD-glucan is a promising immunochemotherapeutic anti-tumor agent.

ISSN 

1019-6439

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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