상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines

Authors 

H B LeeY KimH Z JinJung Joon LeeChang-Jin KimJ Y ParkH S Jung

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

Letters in Applied Microbiology, vol. 40, no. 6, pp. 497-503

Keywords 

cytotoxicityharzianum ahypocreamycotoxintrichodermatumour cell lineinternal transcribed spacertrichothecene derivativeunclassified drugcancer cell culture

Abstract 

Aims: To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines. Methods and Results: A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and 1H and 13C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC50 value of 0.65 and 5.07 μg ml-1 respectively. Conclusions: Harzianum A with a chemical formula of C23H 28O6 was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines. Significance and Impact of the Study: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.

ISSN 

0266-8254

Link 

http://dx.doi.org/10.1111/j.1472-765X.2005.01719.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)