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Title 

Mechanism of natural rifampin resistance of Streptomyces spp.

Authors 

H KimS H KimY H YingH J KimY H KohChang-Jin KimS H LeeC Y ChaY H KookB J Kim

Publisher 

Elsevier

Issue Date 

2005

Citation 

Systematic Applied Microbiology, vol. 28, no. 5, pp. 398-404

Keywords 

rifampin resistant mechanismRpoB genestreptomycesantibiotic resistancedrug resistance, bacterialrifampin

Abstract 

In a previous phylogenetic study of the genus Streptomyces using the rpoB gene, N531, which stands for an aspargine residue in position 531 of RpoB instead of serine (S531), known to be associated with natural rifampin resistance in several organisms, was also observed in the RpoB of several Streptomyces species. To determine whether N531 is associated with the rifampin resistance of Streptomyces strains, we analyzed the rifampin minimum inhibitory concentrations (MICs) of 11 strains of the N531 RpoB type (putative rifampin resistant strains) and of 12 strains of the S531 RpoB type. (putative rifampin susceptible strains). In general, the N531 RpoB types showed higher MIC levels (16-128 μg/ml) than the S531 RpoB types (0-8 μg/ml). To determine the isolation frequencies of N531 RpoB types versus rifampin concentration, we applied screening methods involving different rifampin concentrations (0, 20 and 100 μg/ml) to Korean soils. Higher isolation frequencies of the N531 RpoB types were observed at the higher rifampin concentrations. In addition, during the course of this study we developed an allele specific PCR method to detect rifampin resistant Streptomyces strains. Our results strongly suggested that N531 might be involved in a major mechanism of natural rifampin resistance in strains of the genus Streptomyces.

ISSN 

0723-2020

Link 

http://dx.doi.org/10.1016/j.syapm.2005.02.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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