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Title 

Genetic and sequence analysis of the RAPD fingerprint containing a Rsv3-linked marker rapidly identified by using RILs in soybean

Authors 

Seok YuJ K MoonT Y HwangY H KimHwan Mook KimSoon Chun Jeong

Publisher 

The Genetics Society of Korea

Issue Date 

2005

Citation 

Korean Journal of Genetics, vol. 27, no. 2, pp. 127-132

Keywords 

marker-assisted selectionRAPDSMVsoybeantransposable elementglycine maxsoybean mosaic virus

Abstract 

Soybean mosaic virus (SMV) is a prevalent virus that infects soybean [Glycine max (L.) Merr.] crops worldwide. The Rsv3 gene of soybean confers resistance to three (G5-G7) of the most virulent strains of 7 defined SMV strains, which were grouped based on virus-reaction phenotypes of a spectrum of soybean cultivars mainly carrying Rsv1 alleles. The objective of this study was to develop high throughput molecular markers tightly linked to Rsv3 for marker-assisted selection by the random amplified polymorphic DNA (RAPD) fingerprinting method. A recombinant inbred line (RIL) population from a cross between 'IT182932' and 'Hwangkeumkong' and a BC3F2 population from a cross between 'L29' (resistant) and 'Sowonkong' (susceptible) were primarily used. Two hundred twenty oligonucleotide primers (10mer) were run in a set of recombinant individuals. A polymorphic DNA fragment generated by OP-Z20 was tightly linked to Rsv3 in the analysis of the BC3F 2 population. The fragment was subsequently sequenced and converted to a semi-codominant marker. Sequence analysis of a common band generated by OP-Z20 revealed similarity to the sequence of transposase, characteristic of the Mutator transposable element system. The results could facilitate marker-assisted selection to pyramid Rsv3 with other SMV resistance genes into elite soybean cultivars.

ISSN 

0254-5934

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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