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Title 

Human β-globin second intron highly enhances expression of foreign genes from murine cytomegalovirus immediate-early promoter

Authors 

M KangSeon-Young KimS LeeY K LeeJ LeeH S ShinY S Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2005

Citation 

Journal of Microbiology and Biotechnology, vol. 15, no. 3, pp. 544-550

Keywords 

high-level gene expressionhuman β-globin second intronMCMV IE promotercytomegalovirusgene expressiongene insertiongenetic transfectionhumanhuman cellintron

Abstract 

To develop a highly efficient mammalian expression vector, a series of vectors were constructed based on the murine cytomegalovirus (MCMV) immediate-early (IE) promoter and human β-globin second intron. The resulting MCMV promoter was several-fold stronger than the HCMV promoter in various mammalian cell lines, such as the NIH3T3, Neuro-2a, 293T, and HT1080 cell lines, and was only slightly weaker than the HCMV promoter in HeLa and CHO cells. The inclusion of the human β-globin second intron behind the MCMV promoter or HCMV promoter markedly enhanced the promoter activity in various mammalian cell lines, and the resultant MCMV/Glo-I expression system was stronger than the HCMV promoter from 4.7- to 11.2-fold in every cell line tested. Also, the MCMV/Glo-I promoter induced a higher level of the VSV-G protein in a transiently transfected 293T cell line, which is useful for the production of recombinant retrovirus and lentivirus vectors.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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