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Title 

Generation of subspecies level-specific microbial diagnostic microarrays using genes amplified from subtractive suppression hybridization as microarray probes

Authors 

Jin-Woo BaeS K RheeYoung Do NamYong Ha Park

Publisher 

Oxford University Press

Issue Date 

2005

Citation 

Nucleic Acids Research, vol. 33, no. 13, pp. e113-e113

Keywords 

bacterial genediagnostic procedureDNA microarrayDNA probegeneticsnucleic acid hybridizationDNA probesgenes, bacterialmolecular diagnostic techniques

Abstract 

The generation of microarray probes with specificity below the species level is an ongoing challenge, not least because the high-throughput detection of microorganisms would be an efficient means of identifying environmentally relevant microbes. Here, we describe how suppression subtractive hybridization (SSH) can be applied to the production of microarray probes that are useful for microbial differentiation at the subspecies level. SSH was used to initially isolate unique genomic sequences of nine Salmonella strains, and these were validated in quadruplicate by microarray analysis. The results obtained indicate that a large group of genes subtracted by SSH could serve together, as one probe, for detecting a microbial subspecies. Similarly, the whole microbial genome (not subjected to SSH) can be used as a species-specific probe. The detailed methods described herein could be used and adapted for the estimation of any cultivable bacteria from different environments.

ISSN 

0305-1048

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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