상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

C-terminal serine rich region of IKK-β interacts with Bfl-1, a homologue of Bcl-2

 

Bcl-2 유사인자 Bfl-1과 결합하는 IKK-β의 C말단 Ser부위

Authors 

Hyun Kyung YoonJin Koo KimHee Jun ChoMi Young ChoYuk-Pheel ParkK H YouJ W KimDo Young YoonYong Kyung ChoeHee Gu Lee

Issue Date 

2005

Citation 

Korean Journal of Laboratory Medicine, vol. 25, no. 3, pp. 192-198

Keywords 

Bfl-1IKK- yeast two hybridBH domain

Abstract 

Background : Bcl-2 family proteins play a central role in regulating apoptosis. In human, over 20 members of this family have been identified to date. Bfl-1, a member of the Bcl-2 family, has been known to retard apoptosis in various cell lines. However, the function of Bfl-1 remains unclear. Methods : In order to investigate the Bfl-1 function, we employed yeast two-hybrid system to identify the proteins which are capable of interacting with Bfl-1. The interaction of inhibitor kappaB kinase- (IKK- ) and Bfl-1 was confirmed using glutathione S-transferase pull down assays. To determine which regions of IKK- were required for interaction with Bfl-1, we constructed 12 deletion mutants of IKK- and 5 deletion mutants of Bfl-1. Results : Bfl-1 interacted with the C-terminal region of IKK- which is a subunit of IKK complex, and IKK- activity is very important in the NF- B related pathway. In addition, the amino acids 673-745 of IKK- were important for Bfl-1 interactions, and amino acids 1-484 of Bfl-1, including Bcl-2 homology domains (BH1, BH2, BH3, BH4), were crucial for IKK- interactions. Conclusions : IKK C-terminus contains many serine residues as binding partner of Bfl-1. Our results suggested that Bfl-1 is involved in the NF- B activation through interaction of IKK- and Bfl-1. Further studies need to be performed to understand functions of the IKK- and Bfl-1 associated with the regulation of the NF- B activation pathway.

ISSN 

1598-6535

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)