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Title 

Expression of hepatitis C virus structural proteins in Saccharomyces cerevisiae

Authors 

J S LeeJ YuH J ShinY S KimJ K AhnC K LeeHaryoung PooC J Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2005

Citation 

Journal of Microbiology and Biotechnology, vol. 15, no. 4, pp. 767-771

Keywords 

HCV structural proteinsvaccineyeast expressionglyceraldehyde 3 phosphate dehydrogenasehepatitis C vaccinesucrosevirus proteincontrolled studyexpression vectorgenetic code

Abstract 

Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-E1-E2, c740) fused in-frame with, and immediately 3′ to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/cm3. These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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