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Title 

Inhibition of NF-IL6 activity by manassantin B, a dilignan isolated from Saururus chinensis, in phorbol myristate acetate-stimulated U937 promonocytic cells

Authors 

K N SonI S SongY H ShinT K PaiD K ChungN I BaekJung Joon LeeJ Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2005

Citation 

Molecules and Cells, vol. 20, no. 1, pp. 105-111

Keywords 

C/EBPβinhibitorsmanassantinNF-κBNF-IL6saururus chinensisI kappa Bimmunoglobulin enhancer binding proteinmanassantin Bnuclear factor

Abstract 

Mannasantin B, a dilignan structurally related to manssantin A, is an inhibitor of NF-κB transactivation. In the present study, we found that it inhibited PMA-induced expression of IL-1β, IL-1β mRNA, and IL-1β promoter activity in U937 cells with IC50 values of about 50 nM. It also inhibited NF-IL6- and NF-κB-induced activation of IL-1β, with IC50 values of 78 nM and 1.6 μM, respectively, revealing a potent inhibitory effect on NF-IL6. Electrophoretic mobility shift assays showed that manassantin B had an inhibitory effect on DNA binding by NF-IL6, but not by NF-κB. Further analysis revealed that transactivation by NF-IL6 was also inhibited. Our results indicate that manassantin B suppresses expression of IL-1β in promonocytic cells by inhibiting not only NF-κB but also NF-IL6 activity. Furthermore, our observations suggest that manassantin B may be clinically useful as a potent inhibitor of NF-IL6 activity.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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