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Title 

c-Jun/activator protein-1 mediates interleukin-1β-induced dedifferentiation but not cyclooxygenase-2 expression in articular chondrocytes

Authors 

S G HwangS S YuHaryoung PooJ S Chun

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2005

Citation 

Journal of Biological Chemistry, vol. 280, no. 33, pp. 29780-29787

Keywords 

activation analysiscartilagecollagencytologychondrocyte de differentiationinterleukintranscription factorproteinscollagen type 2cyclooxygenase 2

Abstract 

Interleukin (IL)-1β is a major catabolic pro-inflammatory cytokine involved in cartilage destruction-associated processes, such as loss of the differentiated chondrocyte phenotype (dedifferentiation) and inflammation. Here, we investigated the role of c-Jun and activator protein-1 (AP-1) in IL-1β-induced dedifferentiation and cyclooxygenase (COX)-2 expression in primary cultured chondrocytes. IL-1β induced expression and transient phosphorylation of c-Jun in primary cultured chondrocytes. Ectopic expression of c-Jun was sufficient to cause dedifferentiation, whereas expression of dominant negative c-Jun blocked IL-1β-induced dedifferentiation. Interestingly, modulation of c-Jun expression did not affect IL-1β-induced COX-2 expression. Further experiments revealed that c-Jun phosphorylation was mediated by c-Jun N-terminal kinase and was required for IL-1β-induced dedifferentiation but not COX-2 expression. Consistent with its ability to induce phosphorylation of c-Jun, IL-1β caused transient activation of AP-1, which is necessary for IL-1β-induced dedifferentiation. IL-1β treatment suppressed expression of Sox-9, a major transcription factor that regulates type II collagen expression. Inhibition of c-Jun N-terminal kinase or AP-1 reversed IL-1β-induced suppression of Sox-9, and ectopic expression of c-Jun was sufficient to cause suppression of Sox-9. Our results collectively suggest that IL-1β suppresses type II collagen expression in articular chondrocytes by inducing expression and phosphorylation of c-Jun, AP-1 activation, and subsequent suppression of Sox-9.

ISSN 

0021-9258

Link 

http://dx.doi.org/10.1074/jbc.M411793200

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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