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Title 

Drought-inducible-but ABA-independent-thaumatin-like protein from carrot (Daucus carota L.)

Authors 

Y C JungH J LeeS S YumW Y SohD Y ChoC K AuhT K LeeH C SohY S KimS C Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2005

Citation 

Plant Cell Reports, vol. 24, no. 6, pp. 366-373

Keywords 

daucus carotaDcTLPdroughtthaumatinthaumatin-like proteinproteinsarabidopsis proteinATLP 1 protein, arabidopsisvegetable proteincarrot

Abstract 

Drought treatment induces the accumulation of dcTLP, which is similar in structure to the thaumatin-like proteins (TLPs) found in the embryogenic calli, seedlings, and mature plants of carrot (Daucus carota). We isolated a full-length dcTLP cDNA clone from carrot and characterized the 5′ upstream sequences. The coding region of dcTLP consisted of 645 nucleotides; the theoretical pI value was 4.9, and its molecular weight was approximately 22 kDa. The production of dcTLP transcripts in the seedlings increased dramatically with dehydration treatment but was not affected by abscisic acid (ABA), salicylic acid, or jasmonic acid. The expression patterns of dcTLP mRNA at different developmental stages and in response to a variety of signal molecules was analyzed using reverse transcriptase-PCR and promoter analysis with fused genes of 0.5-kb 5′ upstream sequences in which β-glucuronidase (GUS) reporter genes (gus) were established. The induction of dcTLP was found to be highly specific to drought stress in the embryogenic calli, seedlings, and mature plants. Our results suggest that this new isoform of TLP that has been isolated from carrot is a drought-specific, ABA-independent, non-organ-specific, and non-developmental-stage-specific protein.

ISSN 

0721-7714

Link 

http://dx.doi.org/10.1007/s00299-005-0944-x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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