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Title 

Effects of hydroxyl group numbers on the B-ring of 5,7-Dihydroxyflavones on the differential inhibition of human CYP 1A and CYP1B1 enzymes

 

5,7-Dihydroxyflavones의 CYP 1A and CYP1B1 효소에 대한 영향

Authors 

H J KimS B LeeSong Kyu ParkHwan Mook KimY I ParkM S Dong

Publisher 

Pharmaceutical Society of Korea

Issue Date 

2005

Citation 

Archives of Pharmacal Research, vol. 28, no. 10, pp. 1114-1121

Keywords 

apigeninchrysinCYP1 enzymesethoxyresorufin O-deethylaseluteolincytochrome P 450 CYP1B1cytochrome P-450 CYP1B1cytochrome P450 1A1cytochrome P450 1A2enzyme inhibitor

Abstract 

Flavonoids are polyphenols composed of two aromatic rings (A, B) and a heterocyclic ring (C). In order to determine the effects of the number of hydroxyl groups in the B-ring of the flavonoids on human cytochrome P450 (CYP) 1 family enzymes, we evaluated the inhibition of CYP1A-dependent 7-ethoxyresorufin O-deethylation activity by chrysin, apigenin and luteolin, using bacterial membranes that co-express human CYP1A1, CYP1A2, or CYP1B1 with human NADPH-cytochrome P450 reductase. Chrysin, which possesses no hydroxyl groups in its B-ring, exhibited the most pronounced inhibitory effects on CYP1A2-dependent EROD activity, followed by apigenin and luteolin. On the contrary, CYP1A1-mediated EROD activity was most potently inhibited by luteolin, which is characterized by two hydroxyl groups in its Bring, followed by apigenin and chrysin. However, all of the 5,7-dihydroxyflavones were determined to similarly inhibit CYP1B1 activity. Chrysin, apigenin, and luteolin exhibited a mixedtype mode of inhibition with regard to CYP1A2, CYP1B1, and CYP1A1, with apparent Ki values of 2.4, 0.5, and 2.0 μM, respectively. These findings suggested that the number of hydroxyl groups in the B-ring of 5,7-dihydroxyflavone might have some influence on the degree to which CYP1A enzymes were inhibited, but not on the degree to which CYP1B1 enzymes were inhibited.

ISSN 

0253-6269

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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