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Title 

Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes

Authors 

Deog Bon KooJung IL ChaeJi Su KimGabbine WeeBong Seok SongKyung Kwang LeeYong Mahn Han

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

Molecular Reproduction and Development, vol. 72, no. 4, pp. 542-549

Keywords 

activationMAP kinaseMPFporcine oocytessingle electrical stimulusmitogen activated protein kinaseembryo developmentenzyme inactivationoocyte developmentCDC2 protein kinase

Abstract 

This study was conducted to examine the activities of maturation-promoting factor (MPF) and mitogen-activated protein (MAP) kinase in the porcine oocytes after artificial activation. To determine optimal electrical activation condition, oocytes were exposed to single DC pulse in a variety of electric field strengths (120, 150, 180, and 210 V/mm) and pulse durations (15, 30, 45, and 60 μsec). After the artificial activation, 40-50 oocytes were cultured in a 50 μl drop of NCSU23 medium supplemented with 0.4% BSA at 39°C, 5% CO2 in air for 6 days. No difference was detected in the preimplantation development of pocine oocytes and the mean nuclei number of blastocysts between electric field strengths. Under the 180 V/mm electric field strength, short pulse durations (15 and 30 μsec) showed a higher preimplantation developmental rate of the oocytes and mean nuclei number of blastocysts than an extended electric pulse (60 μsec) (P < 0.05). Single electrical stimulus (180 V/mm, 15 μusec) resulted in higher preimplantation development of porcine oocytes as compared to other chemical stimulators (P < 0.01). Western blot analyses showed the decrease of MPF and MAP kinase in the electrically-activated oocytes. After single electrical stimulus, the amounts of both cdc2 and ERK in porcine oocytes were remarkably reduced by 4 hr and then further decreased by 8 hr. However, the chemically-stimulated oocytes did not show any significant change at the levels of MPF and MAP kinase. Our results indicate that the optimal single electrical pulse is effective on the inactivation of MPF and MAP kinase, eventually leading to the parthenogenetic development of porcine oocytes.

ISSN 

1040-452X

Link 

http://dx.doi.org/10.1002/mrd.20382

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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