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Title 

A new method for the direct application of human plasmin on a dithiothreitol contained agarose stacking gel system

 

인간 플라스민을 위해 DTT를 함유한 새로운 방법의 아가로즈 Stacking 겔

Authors 

Nack Shick ChoiDong Min ChungKab Seog YoonP J MaengSeung Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2005

Citation 

Journal of Biochemistry and Molecular Biology, vol. 38, no. 6, pp. 763-765

Keywords 

diagonal electrophoresisdisulphidedithiothreitolhuman plasminzymography

Abstract 

A new simplified procedure for identifying human plasmin was developed using a DTT copolymerized agarose stacking gel (ASG) system. Agarose (1%) was used for the stacking gel because DTT inhibits the polymerization of acrylamide. Human plasmin showed the lowest activity at pH 9.0. There was a similar catalytically active pattern observed under acidic conditions (pH 3.0) to that observed under alkaline conditions (pH 10.0 or 11.0). Using the ASG system, the primary structure of the heavy chain could be established at pH 3.0. This protein was found to consist of three fragments, 45 kDa, 23 kDa, and 13 kDa. These results showed that the heavy chain has a similar structure to the autolysed plasmin (Wu et al., 1987b) but there is a different start amino acid sequence of the N-termini.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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