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Title 

Estrogen receptor-independent inhibition of tumor necrosis factor-α gene expression by phytoestrogen equol is mediated by blocking nuclear factor-κB activation in mouse macrophages

Authors 

Jong Soon KangYeo Dae YoonMi Hwa HanSang Bae HanKiho LeeMoo Rim KangEun Yi MoonY J JeonSong Kyu ParkHwan Mook Kim

Publisher 

Elsevier

Issue Date 

2005

Citation 

Biochemical Pharmacology, vol. 71, no. 1, pp. 136-143

Keywords 

equolosteoporosisTNF-αNF-κBestrogen receptorhormone receptor blocking agentimmunoglobulin enhancer binding proteinphytoestrogentumor necrosis factor alphacell activation

Abstract 

Equol has been suggested to possess protective effects on bone. However, the underlying mechanism of osteoprotective effect of equol has not been fully understood. In the present study, we examined the effect of equol on tumor necrosis factor-α (TNF-α) gene expression to elucidate a possible mechanism by which equol exerts osteoprotective effect. In vivo administration of equol inhibited TNF-α production by peritoneal macrophages isolated from LPS-treated mice. Equol also dose-dependently inhibited TNF-α production and TNF-α mRNA expression in LPS-stimulated mouse macrophages. Pretreatment of cells with ICI 182.780, an estrogen receptor antagonist, had no effect on the inhibitory efficacy of equol on LPS-induced TNF-α production. Further study demonstrated that equol inhibited NF-κB DNA binding and NF-κB-dependent reporter gene expression in activated RAW 264.7 cells. Moreover, equol blocked degradation of IκBα and IκBβ and nuclear translocation of p65 subunit of NF-κB in activated RAW 264.7 cells. These results suggest that the inhibitory effect of equol on TNF-α expression is mediated, at least in part, by blocking NF-κB activation and the inhibition of TNF-α expression by equol might be involved in its osteoprotective effect.

ISSN 

0006-2952

Link 

http://dx.doi.org/10.1016/j.bcp.2005.10.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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