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Title 

Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria

 

김치 내 유산균 발효과정 모니터링을 위한 Genome-Probing Microarrays(GPM)의 개발

Authors 

Jin-Woo BaeS K RheeJa Ryeong ParkWon Hyong ChungYoung Do NamI LeeH KimYong Ha Park

Publisher 

American Society for Microbiology

Issue Date 

2005

Citation 

Applied and Environmental Microbiology, vol. 71, no. 12, pp. 8825-8835

Keywords 

bacteriagenome-probing microarrays (GPM)microbial genomesbacteriumbacterial genomegenome probing microarraylactic acid bacteriummonitoringoligonucleotide microarrayDNA, bacterial

Abstract 

The genome-probing microarray (GPM) was developed for quantitative, high-throughput monitoring of community dynamics in lactic acid bacteria (LAB) fermentation through the deposit of 149 microbial genomes as probes on a glass slide. Compared to oligonucleotide microarrays, the specificity of GPM was remarkably increased to a species-specific level. GPM possesses about 10- to 100-fold higher sensitivity (2.5 ng of genomic DNA) than the currently used 50-mer oligonucleotide microarrays. Since signal variation between the different genomes was very low compared to that of cDNA or oligonucleotide-based microarrays, the capacity of global quantification of microbial genomes could also be observed in GPM hybridization. In order to assess the applicability of GPMs, LAB community dynamics were monitored during the fermentation of kimchi, a traditional Korean food. In this work, approximately 100 diverse LAB species could be quantitatively analyzed as actively involved in kimchi fermentation.

ISSN 

0099-2240

Link 

http://dx.doi.org/10.1128/AEM.71.12.8825-8835.2005

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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