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Title 

Molecular identification of toxic Alexandrium tamiyavanichii (Dinophyceae) using two DNA probes

Authors 

Choong-Jae KimY Sako

Publisher 

Elsevier

Issue Date 

2005

Citation 

Harmful Algae, vol. 4, no. 6, pp. 984-991

Keywords 

alexandrium tamiyavanichiiDNA probefluorescence in situ hybridization (FISH)paralytic shellfish poisoning (PSP)alexandriumalexandrium catenellaalexandrium fraterculusalexandrium insuetumalexandrium pseudogonyaulaxalexandrium tamarense

Abstract 

To improve labeling-intensity of whole-cell fluorescence in situ hybridization (FISH) in the molecular identification of toxic Alexandrium tamiyavanichii, two DNA probes (TAMID2 plus TAMIS1 designed from the LSU and SSU rDNA regions, respectively) were used to test the labeling intensity of targeted cultured A. tamiyavanichii cells. The cross-reactivity of the DNA probe to natural seawater samples and six Alexandrium species: A. affine, A. catenella, A. fraterculus, A. insuetum, A. pseudogonyaulax and A. tamarense, was also tested. The labeling intensity of the DNA probe TAMID2S1, a combination of two separate probes that target different regions of the rRNA, was 1.7-2.7 times higher than that of the single DNA probe TAMID2. With cultured A. tamiyavanichii cells in the dead growth phase at 30 days, the TAMID2S1 intensity was 1.9 times higher than that of TAMID2. During a 30-day culture, the labeling intensity of A. tamiyavanichii cells hybridized with TAMID2S1 decreased to 49.4% of the original intensity. No cross-reactivity to various microorganisms in natural seawater samples was found. The two DNA probes together, designated as TAMID2S1, readily detected A. tamiyavanichii added to natural seawater samples, even aged cultured cells.

ISSN 

1568-9883

Link 

http://dx.doi.org/10.1016/j.hal.2005.01.003

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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