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Title 

Transcriptome analysis of human gastric cancer

Authors 

Jung Hwa OhJin Ok YangY HahnMi-Rang KimSang-Soon ByunJeo-Jin JeonJeong-Min KimK S SongS M NohS KimHyang Sook YooYong Sung KimNam-Soon Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2005

Citation 

Mammalian Genome, vol. 16, no. 0, pp. 942-945

Keywords 

transcriptomecancer cell culturecancer patientcancer tissuegenetic analysishumanhuman tissuestomach cancerhumans

Abstract 

To elucidate the genetic events associated with gastric cancer, 124,704 cDNA clones were collected from 37 human gastric cDNA libraries, including 20 full-length enriched cDNA libraries of gastric cancer cell lines and tissues from Korean patients. An analysis of the collected ESTs revealed that 97,930 high-quality ESTs coalesced into 13,001 clusters, of which 11,135 clusters (85.6%) were annotated to known ESTs. The analysis of the full-length cDNAs also revealed that 4862 clusters (51.7%) contained at least one putative full-length cDNA clone with an initiation codon, with the average length of the 5′ UTR of 140 bp. A large number appear to have a diverse transcription start site (TSS). An examination of the TSS of some genes, such as TEGT and GAPD, using 5′ RACE revealed that the predicted TSSs are actually found in human gastric cancer cells and that several TSSs differ depending on the specific gastric cell line. Furthermore, of the human gastric ESTs, 766 genes (9.5%) were present as putative alternatively spliced variants. Confirmation of the predicted spliced isoforms using RT-PCR showed that the predicted isoforms exist in gastric cancer cells and some isoforms coexist in gastric cell lines. These results provide potentially useful information for elucidating the molecular mechanisms associated with gastric oncogenesis.

ISSN 

0938-8990

Link 

http://dx.doi.org/10.1007/s00335-005-0075-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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