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Title 

Methylation changes of lysine 9 of histone H3 during preimplantation mouse development

 

착상전 생쥐 발생동안 히스톤 H3의 9번째 라이신 자닉의 메틸화 변화

Authors 

Seungeun YeoKyung Kwang LeeYong Mahn HanYong-Kook Kang

Publisher 

Springer Verlag (Germany)

Issue Date 

2005

Citation 

Molecules and Cells, vol. 20, no. 3, pp. 423-428

Keywords 

active demethylationDNA methylationepigenetic reprogramminghistone methylationpreimplantation embryopronucleushistone H3histone lysine methyltransferaselysinehistone

Abstract 

Immediately after fertilization, a chromatin remodeling process in the oocyte cytoplasm extracts protamine molecules from the sperm-derived DNA and loads histones onto it. We examined how the histone H3-lysine 9 methylation system is established on the remodeled sperm chromatin in mice. We found that the paternal pronucleus was not stained for dimethylated H3-K9 (H3-m 2K9) during pronucleus development, while the maternal genome stained intensively. Such H3-m2K9 asymmetry between the parental pronuclei was independent of HP1β localization and, much like DNA methylation, was preserved to the two-cell stage when the nucleus appeared to be compartmentalized for H3-m2K9. A conspicuous increase in H3-m 2K9 level was observed at the four-cell stage, and then the level was maintained without a visible change up to the blastocyst stage. The behavior of H3-m2K9 was very similar, but not identical, to that of 5-methylcytosine during preimplantation development, suggesting that there is some connection between methylation of histone and of DNA in early mouse development.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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