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Title 

Dehydrotrametenolic acid selectively inhibits the growth of H-ras transformed rat2 cells and induces apoptosis through caspase-3 pathway

 

Dehydrotrametenolic acid의 세포사멸 유도를 통한 항암효과

Authors 

Hyun-Mi KangSu-Kyung LeeDae Seop ShinMi-Young LeeDong Cho HanN I BaekKwang Hee SonByoung-Mog Kwon

Publisher 

Elsevier

Issue Date 

2006

Citation 

Life Sciences, vol. 78, no. 6, pp. 607-613

Keywords 

apoptosiscaspase-3cell cycle arrestdehydrotrametenolic acidH-rasporia cocos wolfcaspase 3animal cellcell cycle G1 phase

Abstract 

The screening of natural products that preferentially inhibit growth of H-ras transformed rat2 cells vs. rat2 cells was performed to identify H-ras specific growth inhibitor. A lanostane-type triterpene acid, dehydrotrametenolic acid (3β-hydroxylanosta-7,9(11),24-trien-21-oic acid), was isolated from the sclerotium of Poria cocos (Polyporaceae). Dehydrotrametenolic acid selectively inhibited the growth of H-ras transformed cells with a GI 50 value of 40 μM. FACS analysis indicated that the compound exerted its anti-proliferation effects through cell cycle arrest at G2/M phase and accumulation of sub-G1 population. Dehydrotrametenolic acid-induced apoptosis was further confirmed with chromosomal DNA fragmentation, caspase-3 activation, and degradation of PARP and Lamin A/C degradation. The compound also regulated the expression of H-ras, Akt and Erk, which are the downstream proteins of H-ras signaling pathways. The results suggest that dehydrotrametenolic acid can be a potential anticancer agent against H-ras transformed tumor.

ISSN 

0024-3205

Link 

http://dx.doi.org/10.1016/j.lfs.2005.05.066

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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