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Title 

Cyanobacterial diversity analysis using cpcBA-Intergenic spacer region

 

cpcBA-intergenic spacer region을 이용한 cyanobacteria의 다양성 분석

Authors 

Gang Guk ChoiYong Ha ParkChi-Yong AhnMyoung Sook BaeHee-Mock Oh

Publisher 

The Microbiological Society of Korea

Issue Date 

2005

Citation 

Korean Journal of Microbiology, vol. 41, no. 4, pp. 287-292

Keywords 

community analysiscpcBA-IGScyanobacteriadiversityPCR-RFLP

Abstract 

The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified cpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005. The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBAIGS can be used for the species identification and community analysis of cyanobacteria.

ISSN 

0440-2413

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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