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Title 

Temperature effect on the functional expression of human cytochromes P450 2A6 and 2E1 in Escherichia coli

Authors 

S K YimT AhnHeung Chae JungJae Gu PanC H Yun

Publisher 

Pharmaceutical Society of Korea

Issue Date 

2005

Citation 

Archives of Pharmacal Research, vol. 28, no. 4, pp. 433-437

Keywords 

CYP expressionhuman CYP2A6human CYP2E1temperature effectCYP2F1 protein, humancytochrome P450escherichia colihumantemperaturecytochrome P-450 enzyme system

Abstract 

Human cytochromes P450 (CYP) 2A6 and 2E1 are of great interest because of their important roles in the oxidation of numerous drugs and carcinogens. Bacterial expression systems, especially Escherichia coli cells, have been widely used for the production of various CYP enzymes in order to obtain high yield of proteins. The expression methods usually employ longer culture time (30-72 h) at lower temperature (usually under 30°C). Expression levels of CYPs 2A6 and 2E1 at 37°C were compared to those at 28°C, which is a usual temperature used in most bacterial expression systems for human CYP expression. Within 18 h the expression levels of CYPs 2A6 and 2E1 reached up to 360 and 560 nmol per liter culture at 37°C, respectively, which are compatible with those of 36 h culture at 28°C. The activities of CYPs expressed at 37°C were also comparable to those expressed at 28°C. The present overexpression system can be useful for rapid production of large amounts of active human CYPs 2A6 and 2E1 in E. coli.

ISSN 

0253-6269

Link 

http://dx.doi.org/10.1007/BF02977672

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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