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Title 

Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner

Authors 

J P ParkK B LeeS J LeeT J ParkMin-Gon KimBong Hyun ChungZ W LeeI S ChoiS Y Lee

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

Biotechnology and Bioengineering, vol. 92, no. 2, pp. 160-165

Keywords 

microcontact printingPHApolyhydroxyalkanoatessubstrate binding domainsurface plasmon resonanceantibodiesbinding energybioassayproteins

Abstract 

A novel strategy for micropatterning proteins on the surface of polyhydroxyalkanoate (PHA) biopolymer by microcontact printing (μCP) is described. The substrate binding domain (SBD) of the Pseudomonas stutzeri PHA depolymerase was used as a fusion partner for specifically immobilizing proteins on PHA substrate. Enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) fused to the SBD could be specifically immobilized on the micropatterns of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3- hydroxyhexanoate). Laser scanning confocal microscopic studies suggested that two fusion proteins were micropatterned in their functionally active forms. Also, antibody binding assay by surface plasmon resonance suggested that protein-protein interaction studies could be carried out using this system.

ISSN 

0006-3592

Link 

http://dx.doi.org/10.1002/bit.20581

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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