상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Molecular and biochemical analysis of phthalate and terephthalate degradation by Rhodococcus sp. strain DK17

Authors 

K Y ChoiDockyu KimW J SulJ C ChaeG J ZylstraY M KimE Kim

Publisher 

Wiley-Blackwell

Issue Date 

2005

Citation 

FEMS Microbiology Letters, vol. 252, no. 2, pp. 207-213

Keywords 

gene duplicationphthalate/terephthalate operonprotocatechuate 3,4-dioxygenaserhodococcus1,2 dihydro 1,2 dihydroxyterephthalate3,4 dihydro 3,4 dihydroxyphthalatebacterial straindegradationsequence analysisbiodegradation, environmental

Abstract 

Alkylbenzene-degrading Rhodococcus sp. strain DK17 is able to utilize phthalate and terephthalate as growth substrates. The genes encoding the transformation of phthalate and terephthalate to protocatechuate are organized as two separate operons, located 6.7 kb away from each other. Interestingly, both the phthalate and terephthalate operons are induced in response to terephthalate while expression of the terephthalate genes is undetectable in phthalate-grown cells. In addition to two known plasmids (380-kb pDK1 and 330-kb pDK2), a third megaplasmid (750-kb pDK3) was newly identified in DK17. The phthalate and terephthalate operons are duplicated and are present on both pDK2 and pDK3. RT-PCR experiments, coupled with sequence analysis, suggest that phthalate and terephthalate degradation in DK17 proceeds through oxygenation at carbons 3 and 4 and at carbons 1 and 2 to form 3,4-dihydro-3,4- dihydroxyphthalate and 1,2-dihydro-1,2-dihydroxyterephthalate, respectively. The 3,4-dihydroxyphthalate pathway was further corroborated through colorometric tests. Apparently, the two dihydrodiol metabolites are subsequently dehydrogenated and decarboxylated to form protocatechuate, which is further degraded by a protocatechuate 3,4-dioxygenase as confirmed by a ring-cleavage enzyme assay.

ISSN 

0378-1097

Link 

http://dx.doi.org/10.1016/j.femsle.2005.08.045

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)