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Title 

High-Level production of low-branched levan from Pseudomonas aurantiaca S-4380 for the production of di-β-D-Fructofuranose dianhydride IV

 

Pseudomonas aurantiaca S-4380을 사용한 di-β-D-F

Authors 

K H JangEun-Kyung JangS H KimI H KimS A KangIssac KohY I ParkY J KimS D HaChul Ho Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2006

Citation 

Journal of Microbiology and Biotechnology, vol. 16, no. 1, pp. 102-108

Keywords 

di-β-D-fructofuranose dianhydride IVlevanpseudomonas aurantiaca S-4380fructosegenomic DNAinulinaselevansucrasebacterial straincarbohydrate synthesiscross linking

Abstract 

The lscA gene, encoding a levansucrase of 424 amino acids (aa) residues, was cloned from the genomic DNA of Pseudomonas aurantiaca S-4380, and overexpressed in Escherichia coli. The recombinant levansucrase overexpressed in E. coli was then used to produce levan from sucrose. Levan crystals with 98% purity could be obtained from the reaction mixture with 62% yield using an alcohol precipitation method. The molecular weight of the levan was 7×105 daltons. Methylation studies showed that the levan was branched: main linkage C-2,6; branched linkage C-2,1; and degree of branching 6%. Three bacterial levans from different strains were incubated with levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032, which produced di-β-D-fructofuranose dianhydride IV (DFA IV); final conversion yields from the levans to DFA IV were 39% in Zymomonas mobilis, 53% in Serratia levanicum, and 59% in P. aurantiaca S-4380 levansucrase. The levan from P. aurantiaca S-4380 levansucrase gave the highest conversion yield of levan to DFAIV so far reported.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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