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Title 

TEM sample preparation for cultured neurons on a glass coverslip

 

Hydrofluoric acid 용액을 이용한 유리 커버글라스에 배양된 신경세포의 전자현미경 시료제작법

Authors 

Hyun Woo OhHo Yong Park

Issue Date 

2005

Citation 

Korean Journal of Electron Microscopy, vol. 35, no. 4, pp. 197-202

Keywords 

cell cultureglass coversliphydrofluoric acidsynapse

Abstract 

Cultured neurons from Drosophila brain on a glass coverslip to understand the structural basis of synapse were prepared for TEM observations. Neurons on a coverslip were fixed, dehydrated and embedded in Epon without separating from coverslip. After polymerization, the block was placed in 49% hydrofluoric acid to remove the coverslip. The block was examined under a light microscope to select exact neurons, then trimmed and sectioned for TEM observation.

ISSN 

1225-6773

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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