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Title 

Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration

Authors 

Yong Sam KimHye Yeon KangJ Y KimS OhC H KimChun Jeih RyuE MiyoshiN TaniguchiJeong Heon Ko

Publisher 

Wiley-Blackwell

Issue Date 

2006

Citation 

Proteomics, vol. 6, no. 4, pp. 1187-1191

Keywords 

2-DEcolon cancerglycomicsN-Acetylglucosaminyl transferase Vprotein tyrosine phosphatase κalpha 1,3(6) mannosylglycoprotein beta 1,6 n acetylglucosaminyltransferaselectinn acetylglucosaminyltransferaseprotein tyrosine phosphataseprotein tyrosine phosphatase kappa

Abstract 

To gain a better understanding of the mechanism underlying colon cancer and to search for potential markers of colon cancer prognosis, a comparative proteomic analysis of colon cancer WiDr cells was conducted using 2-DE and lectin blot, followed by identification based on ESI-MS. Through these approaches 14 proteins were identified as candidate target proteins for N-acetylglucosaminyl transferase V (GnT-V) that would be expected to be implicated in the progression of colon cancer. We selected protein tyrosine phosphatase kappa (PTPκ) as a model protein to validate this approach to the discovery of novel biomarkers in colon cancer. PTPκ underwent an aberrant glycosylation in GnT-V-overexpressing WiDr cells, and the aberrantly glycosylated PTPκ was vulnerable to proteolytic cleavage. The enhanced cleavage of PTPκ in GnT-V-overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.200500400

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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