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Title 

Enzymatic synthesis of ascorbic acid fructoside by transfructosylation using levan fructotransferase

Authors 

Choong Yeul LeeKi Ho KimSun Yeon HurJoo-Hyung HeoMin Ho ChoiSang Ki RheeChul Ho Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2006

Citation 

Journal of Microbiology and Biotechnology, vol. 16, no. 1, pp. 64-67

Keywords 

ascorbic acid 2-fructosidefructosideglycosideglycosylationlevan fructotransferaseascorbic acid 2 fructosideascorbic acid derivativelevansucrasedrug synthesis

Abstract 

To enhance the stability of ascorbic acid, the glycosylation of ascorbic acid was studied using the transfructosylation activity of levan fructotransferase. When levan was used as glycosyl donor, a novel fructoside (ascorbic acid 2-fructoside) was formed by the transfructosylation activity of the levan fructotransferase. The production of ascorbic acid 2-fructoside was highly affected by the concentration of the fructosyl acceptor (ascorbic acid). When 35% of ascorbic acid and 2% of levan were incubated with LFTase of 0.5 unit/g-levan at 37°C for 85 h, a maximum 52 g/l of AA-2F was produced.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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