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Title 

Production of human lactoferrin in transgenic cell suspension cultures of sweet potato

Authors 

Sung Ran MinJ W WooWon Joong ChungS K HanY B LeeJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2006

Citation 

Biologia Plantarum, vol. 50, no. 1, pp. 131-134

Keywords 

genetic transformationipomoea batatasparticle bombardment

Abstract 

Shoot apical meristem-derived calli were transformed with a hLF cDNA in an attempt to produce human lactoferrin (hLF) in transgenic cell suspension cultures of sweet potato [Ipomoea batatas (L.) Lam.]. Calli were bombarded with tungsten particles coated with the binary vector pLSM1 containing a hLF cDNA under the control of the 35S promoter and the neomycin phosphotransferase gene as a selection marker. Calli were then transferred to Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 100 mg dm-3 kanamycin. Kanamycin-resistant calli were selected at four-week intervals and subcultured. Cell suspension cultures were established in liquid MS medium with 4.52 μM 2,4-D. Southern and Northern blot analyses confirmed that hLF cDNA was incorporated into the plant genome and was properly expressed in the cells. ELISA analysis showed that transgenic cells produced hLF up to 3.2 μg mg-1 (total protein).

ISSN 

0006-3134

Link 

http://dx.doi.org/10.1007/s10535-005-0087-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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