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Title 

Confirmation of a linkage between H-Ras and MMP-13 expression as well as MMP-9 by chemical genomic approach

 

화학유전체 연구방법을 이용한 라스 활성과 신생혈관 형성과의 관계 규명

Authors 

Su Kyung LeeJung Min KimMi Young LeeKwang Hee SonYoung Il YeomC H KimY ShinJ S KohDong Cho HanByoung-Mog Kwon

Publisher 

Wiley-Blackwell

Issue Date 

2006

Citation 

International Journal of Cancer, vol. 118, no. 9, pp. 2172-2181

Keywords 

cDNA microarrayfarnesyl transferasegene expression profileH-RasMMPs chemical genomics1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazoleanthra[1,9 cd]pyrazol 6(2h) onearteminolidecollagenase 3

Abstract 

As farnesylation of the Ras protein by farnesyl transferase is a critical step for the Ras functional activity, the farnesyl transferase inhibitor could affect H-Ras functions and the inhibitors such as arteminolide, SCH66336 and LB42908 completely inhibited Ras-farnesylation. However, they did not induce apoptosis of H-Ras-transformed cells with concentration for blocking H-Ras farnesylation. To determine the antitumor effects of the inhibitors, it was analyzed through the expression profile of genes, regulated by activated H-Ras or FTIs by using cDNA microarray. On the basis of the results, the relationship between H-Ras and MMPs expression was confirmed by RT-PCR, Western bolt, zymographic analysis and angiogenesis assay. Our results suggested that activation of MMP-13 as well as MMP-9 induced by H-Ras is involved in angiogenesis and with farnesyl transferase inhibitors, in part, exerts their anticancer effects. We confirmed that MMP-13 is a critical H-Ras target gene through chemical genomic approaches with farnesyl transferase inhibitors.

ISSN 

0020-7136

Link 

http://dx.doi.org/10.1002/ijc.21610

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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