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Title 

Changes in lipoxygenase properties and activity related to postgerminative growth and senescence in oat (Avena sativa L. cv. Victory 1)

Authors 

Hoonbok YiM A YiH T Choi

Publisher 

The Botanical Society of Korea

Issue Date 

2005

Citation 

Journal of Plant Biology, vol. 48, no. 4, pp. 429-439

Keywords 

isoelectric focusinglipoxygenaseoatspolyacrylamide gel electrophoresisseedlingsseeds

Abstract 

Lipoxygenase (LOX), one of the main oxidative catalysts in plants, is involved in the regulation of growth and senescence. We investigated changes in LOX activity or its properties as they related to the development of oat plants at four stages (germination, growth, natural senescence, and dark-incubated senescence). LOX activity was high during early growth and at senescence. At pH 4.5, activity showed an abrupt surge compared with a normal enzyme reaction pattern at pH 6.5. The optimum reaction temperature was 25°C; LOX and peroxidase exhibited similar activity patterns. Polyacrylamide gel electrophoresis revealed that the purified LOX consisted of three isoenzymes in germinating seeds, two in growing seedlings, and three during both natural and dark-induced senescence. As determined by isoelectric focusing, the isoelectric points (pl) of LOX ranged from 3.6 to 6.5 throughout the four developmental stages; for natural or dark-induced senescence, the pl was 9.0.

ISSN 

1226-9239

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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