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Title 

Comparative microarray analysis of programmed cell death induced by proteasome malfunction and hypersensitive response in plants

Authors 

Moonil KimSanghyeob LeeKyoungsook ParkEun-Ju JeongChoong-Min RyuDoil ChoiH S Pai

Publisher 

Elsevier

Issue Date 

2006

Citation 

Biochemical and Biophysical Research Communications, vol. 342, no. 2, pp. 514-521

Keywords 

α6 Subunit of 20S proteasomecDNA microarraygene expressionhot pepperhypersensitive responsevirus-induced gene silencingproteasomecell deathDNA microarraymicroarray analysis

Abstract 

Programmed cell death (PCD) plays a pivotal role in the elimination of injured or unwanted cells during diverse physiological and developmental conditions in organisms. However in contrast to the animal system, signaling pathways and molecular mechanism of PCD are largely unknown in plants. We previously reported that silencing of NbPAF encoding the α6 subunit of 20S proteasome by virus-induced gene silencing activated programmed cell death in plants by inactivating proteasome function. In this study, we analyzed global gene expression profile of PCD induced by suppression of NbPAF expression, in comparison with that of hypersensitive response (HR)-induced PCD, using a cDNA microarray representing 4685 hot pepper genes. HR is a well-characterized PCD program in plants, which occurs in response to pathogen infection. The microarray analyses identified 247 genes whose gene expression was differentially modulated during PCD activated by NbPAF depletion or HR. Most of the genes that were up-regulated during the NbPAF-mediated PCD, including the ubiquitin/proteasome pathway-related genes, were down-regulated during HR cell death. In contrast, transcription of many defense-related genes, transcription factor genes, and photosynthesis-related genes remained unchanged or repressed during NbPAF-mediated PCD, while it was highly induced during HR cell death. Only a small number of genes including antioxidant-related genes and proteases were found to be up-regulated during induction of PCD by both proteasome inactivation and HR. Based on these results, these two PCD pathways appear to be differentially regulated, but some overlapping mechanism exists, which involves core regulators of plant PCD.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2006.01.176

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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