상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Improved L-threonine production of Escherichia coli mutant by optimization of culture conditions

Authors 

M-H LeeHong-Weon LeeJ-H ParkJungoh AhnJoon-Ki JungY-I Hwang

Publisher 

Elsevier

Issue Date 

2006

Citation 

Journal of Bioscience and Bioengineering, vol. 101, no. 2, pp. 127-130

Keywords 

biotinescherichia coli MT201l-methioninel-threonineminimal salt mediumcell cultureoptimizationsaltssynthesis (chemical)escherichia coli

Abstract 

l-Threonine production was investigated in a minimal salt medium using l-threonine-overproducing Escherichia coli MT201, derived from E. coli K-12. It was observed that dry cell weight reached 12.5 g/l with 15.9 g/l l-threonine. To increase dry cell weight and l-threonine production, the fermentation process was optimized. When biotin was added as growth factor, l-threonine production reached 52.0 g/l from 15.9 g/l without biotin. Dry cell weight and l-threonine production were further increased by continuous feeding of the feed media with an optimized l-methionine concentration (5.0 g/l). However, high-cell-density culture caused oxygen-limited condition, which resulted in the accumulation of organic acids. To overcome this problem, oxygen-enriched air was supplied to the fermentor with the minimal salt medium. Under these optimal conditions, we achieved an l-threonine production of 80.2 g/l in the minimal salt medium.

ISSN 

1389-1723

Link 

http://dx.doi.org/10.1263/jbb.101.127

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)