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Title 

Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53

 

HPV, E6, E6AP, p53 상호작용 연구를 위한 SPR 이미징 단백질 어레이

Authors 

H-S RoByung Hoon KohSun Ok JungHyun Kyu ParkYong Beom ShinMin-Gon KimBong Hyun Chung

Publisher 

Wiley-Blackwell

Issue Date 

2006

Citation 

Proteomics, vol. 6, no. 7, pp. 2108-2111

Keywords 

human papillomavirus (HPV)protein arraysprotein interactionsurface plasmon resonance imaginghybrid proteinprotein E6protein E6APprotein p53ubiquitin protein ligasemicroarray analysis

Abstract 

We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HPV) is known to be a causative agent of cervical cancer. Upon infection, the viral E6 protein forms a heterotrimeric protein complex with p53 and E6AP. The formation of the complex eventually results in the degradation of p53. In the present study, a GST-fused E6AP protein was layered onto a glutathione (GSH)-modified gold chip surface. The specific binding of GST-E6AP protein onto the gold chip surface was facilitated through the affinity of GST to its specific ligand GSH. The interacting proteins (E6 and/or p53) were then spotted. Detection of the interaction was performed using a SPR imaging (SPRI) technique. The resulting SPRI intensity data showed that the protein-protein interactions of E6AP, E6, and p53 were detected in a concentration-dependent manner, suggesting that the SPRI-based microarray system can be an effective tool to study protein-protein interactions where multiple proteins are involved.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.200500635

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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