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Title 

Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice

 

사스 바이러스 유산균 점막 백신

Authors 

J S LeeHaryoung PooD P HanS P HongK KimM W ChoE KimM H SungC J Kim

Publisher 

American Society for Microbiology

Issue Date 

2006

Citation 

Journal of Virology, vol. 80, no. 8, pp. 4079-4087

Keywords 

bacterial proteinhybrid proteinimmunoglobulin G antibodyneutralizing antibodypoly gamma glutamic acid synthetase A proteinrecombinant proteinvirus spike proteinantibody responseimmunizationlactobacillus casei

Abstract 

Induction of mucosal immunity may be important for preventing SARS-CoV infections. For safe and effective delivery of viral antigens to the mucosal immune system, we have developed a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (PgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of PgsA and the Spike (S) protein segments SA (residues 2 to 114) and SB (residues 264 to 596) were stably expressed in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses, immunofluorescence microscopy, and flow cytometry. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by enzyme-linked immunosorbent assays using S protein peptides. More importantly, these antibodies exhibited potent neutralizing activities against severe acute respiratory syndrome (SARS) pseudoviruses. Orally immunized mice mounted a greater neutralizing-antibody response than those immunized intranasally. Three new neutralizing epitopes were identified on the S protein using a peptide neutralization interference assay (residues 291 to 308, 520 to 529, and 564 to 581). These results indicate that mucosal immunization with recombinant L. casei expressing SARS-associated coronavirus S protein on its surface provides an effective means for eliciting protective immune response against the virus.

ISSN 

0022-538X

Link 

http://dx.doi.org/10.1128/JVI.80.8.4079-4087.2006

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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