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Title 

Determination of cyanobacterial diversity during algal blooms in Daechung Reservoir, Korea, on the basis of cpcBA Intergenic spacer region analysis

 

cpcBA IGS를 이용하여 대청호에서 수화발생시 남세균

Authors 

Song-Gun KimS K RheeChi-Yong AhnSo-Ra KoGang Guk ChoiJin-Woo BaeY H ParkHee-Mock Oh

Publisher 

American Society for Microbiology

Issue Date 

2006

Citation 

Applied and Environmental Microbiology, vol. 72, no. 5, pp. 3252-3258

Keywords 

algal bloomscyanobacterialspacer DNAalgal bloomKoreaDNA, intergenicsequence analysis, DNAdaechung reservoirsouth KoreaTaejon [South Korea]

Abstract 

The detection and prevention of cyanobacterial blooms are important issues in water quality management. As such, the diversity and community dynamics of cyanobacteria during cyanobacterial bloom in the Daechung Reservoir, Korea, were studied by analyzing the intergenic spacer (IGS) region between phycocyanin subunit genes cpcB and cpcA (cpcBA IGS). To amplify the cpcBA IGS from environmental samples, new PCR primers that could cover a wider range of cyanobacteria than previously known primers were designed. In the samples taken around the bloom peak (2 September 2003), seven groups of cpcBA IGS sequences were detected, and none of the amplified cpcBA IGSs was closely related to the cpcBA IGS from chloroplasts. Apart from the Microcystis-, Aphanizomenon (Anabaena)-, Pseudanabaena-, and Planktothrix (Oscillatoria)-like groups, the three other groups of cpcBA IGS sequences were only distantly related to previously reported sequences (< 85% similarity to their closest relatives). The most prominent changes during the bloom were the gradual decrease and eventual disappearance of the Aphanizomenon (Anabaena)-like group before the bloom peak and the gradual increase and sudden disappearance of Planktothrix (Oscillatoria)-like groups right after the bloom peak. The community succession profile obtained based on the cpcBA IGS analysis was also supported by a PCR-denaturing gradient gel electrophoresis analysis of the 16S rRNA genes.

ISSN 

0099-2240

Link 

http://dx.doi.org/10.1128/AEM.72.5.3252-3258.2006

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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