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Title 

Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles

Authors 

Jinyoung JeongTai-Hwan HaBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2006

Citation 

Analytica Chimica Acta, vol. 569, no. 0, pp. 203-209

Keywords 

enzyme immobilizationgold-coated magnetic nanoparticle (GMP)hexa-arginine-tagged esterase (Arg6-esterase)magnetic separationgold compoundsmagnetic propertiesmercapto-hexadecanoic acid (MHA)argininearginine esterase

Abstract 

Gold-coated magnetic nanoparticle (GMP) was manufactured and used as a carrier for the immobilization of hexa-arginine-tagged esterase (Arg6-esterase). The formation of gold shell on the magnetic nanoparticle (Fe2O3) was performed by an iterative reduction method using hydroxylamine as a reductant. The surface of the GMP was further functionalized with mercapto-hexadecanoic acid (MHA) to tether the positively charged Arg6-esterase effectively. The enzymatic activity of the immobilized Arg6-esterase was investigated by monitoring the dissociation rate of its colorimetric substrate, p-nitrophenol butyrate (pNPB). Although the immobilized enzyme exhibited a lower activity (∼60%) compared to the free one, its original activity was found to be retained even after seven times repetitive uses by magnetic decantation.

ISSN 

0003-2670

Link 

http://dx.doi.org/10.1016/j.aca.2006.03.102

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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