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Title | Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles |
Authors | Jinyoung Jeong; Tai-Hwan Ha; Bong Hyun Chung |
Publisher | Elsevier |
Issue Date | 2006 |
Citation | Analytica Chimica Acta, vol. 569, no. 0, pp. 203-209 |
Keywords | enzyme immobilization; gold-coated magnetic nanoparticle (GMP); hexa-arginine-tagged esterase (Arg6-esterase); magnetic separation; gold compounds; magnetic properties; mercapto-hexadecanoic acid (MHA); arginine; arginine esterase |
Abstract | Gold-coated magnetic nanoparticle (GMP) was manufactured and used as a carrier for the immobilization of hexa-arginine-tagged esterase (Arg6-esterase). The formation of gold shell on the magnetic nanoparticle (Fe2O3) was performed by an iterative reduction method using hydroxylamine as a reductant. The surface of the GMP was further functionalized with mercapto-hexadecanoic acid (MHA) to tether the positively charged Arg6-esterase effectively. The enzymatic activity of the immobilized Arg6-esterase was investigated by monitoring the dissociation rate of its colorimetric substrate, p-nitrophenol butyrate (pNPB). Although the immobilized enzyme exhibited a lower activity (∼60%) compared to the free one, its original activity was found to be retained even after seven times repetitive uses by magnetic decantation. |
ISSN | 0003-2670 |
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Appears in Collections | |
Registered Date |
2017-04-19 |
