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Title 

AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants

Authors 

C H KangW Y JungY H KangJ Y KimD G KimJ C JeongD W BaekJ B JinJ Y LeeM O KimW S ChungT MengisteH KoiwaSang Soo KwakJ D BahkS Y LeeJ S NamD J YunM J Cho

Publisher 

Nature Publishing Group

Issue Date 

2006

Citation 

Cell Death and Differentiation, vol. 13, no. 1, pp. 84-95

Keywords 

arabidopsisBAG domaincalciumcalmodulinCaM-binding proteinIQ motifstressamino acidBAG 6 proteincalcium ion

Abstract 

Calmodulin (CaM) influences many cellular processes by interacting with various proteins. Here, we isolated AtBAG6, an Arabidopsis CaM-binding protein that contains a central BCL-2-associated athanogene (BAG) domain. In yeast and plants, overexpression of AtBAG6 induced cell death phenotypes consistent with programmed cell death (PCD). Recombinant AtBAG6 had higher affinity for CaM in the absence of free Ca2+ than in its presence. An IQ motif (IQXXXRGXXXR, where X denotes any amino-acid) was required for Ca2+-independent CaM complex formation and single amino-acid changes within this motif abrogated both AtBAG6-activated CaM-binding and cell death in yeast and plants. A 134-amino-acid stretch, encompassing both the IQ motif and BAG domain, was sufficient to induce cell death. Agents generating oxygen radicals, which are known to be involved in plant PCD, specifically induced the AtBAG6 transcript. Collectively, these results suggest that AtBAG6 is a stress-upregulated CaM-binding protein involved in plant PCD.

ISSN 

1350-9047

Link 

http://dx.doi.org/10.1038/sj.cdd.4401712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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