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Title 

Reactive oxygen species augment B-cell-activating factor expression

Authors 

Eun Yi MoonJun Hee LeeS Y OhS K RyuHwan Mook KimH S KwakWoon Kee Yoon

Publisher 

Elsevier

Issue Date 

2006

Citation 

Free Radical Biology and Medicine, vol. 40, no. 12, pp. 2103-2111

Keywords 

BAFFLPSnull micePrxIIROSspleen cellsTLR4B cell activating factorI kappa B alphaimmunoglobulin enhancer binding protein

Abstract 

B-cell-activating factor (BAFF) plays a role in mature B-cell generation and maintenance. Lipopolysaccharide (LPS) activates toll-like receptor 4 (TLR4)-dependent signal transduction and induces ROS production. Here, we investigated BAFF production regulated by reactive oxygen species (ROS). BAFF expression was augmented by LPS stimulation and by serum deprivation that induced ROS production. BAFF expression was inhibited by treatment with various antioxidants including N-acetyl-L-cysteine (NAC). We also investigated BAFF expression in vivo using peroxiredoxin II (PrxII)-deficient mouse spleen cells. PrxII is a member of the antioxidant enzyme family that protects cells from oxidative damage. Constitutive production of endogenous ROS was detected in spleen cells lacking PrxII. Serum BAFF protein level and BAFF transcript expression in splenocytes were significantly higher in PrxII-/- mice than wildtype mice. A higher BAFF level is consistent with the higher total number of splenocytes and B220+cells. Results were supported by NF-κB activation as judged by reduced IκBα degradation and increased nuclear translocation of p65/RelA with LPS stimulation, serum deprivation, and PrxII deletion. Data suggest that TLR4-mediated BAFF expression was increased by ROS and it was inhibited by PrxII controlling ROS production.

ISSN 

0891-5849

Link 

http://dx.doi.org/10.1016/j.freeradbiomed.2006.02.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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