상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Over-production of β-carotene from metabolically engineered Escherichia coli

Authors 

S W KimJ B KimW H JungJ H KimJoon-Ki Jung

Publisher 

Springer Verlag (Germany)

Issue Date 

2006

Citation 

Biotechnology Letters, vol. 28, no. 12, pp. 897-904

Keywords 

β-Carotenemetabolic engineeringover-productionsynthetic operonescherichia colibeta carotenebeta Carotene

Abstract 

To produce recombinant β-carotene in vitro, synthetic operons encoding genes governing its biosynthesis were engineered into Escherichia coli. Constructs harboring these operons were introduced into either a high-copy or low-copy cloning vector. β-Carotene production from these recombinant E. coli cells was either constitutive or inducible depending upon plasmid copy number. The most efficient β-carotene production was with the low-copy based vector. The process was increased incrementally from a 5 l to a 50 l fermentor and finally into a 300 l fermentor. The maximal β-carotene yields achieved using the 50 l and 300 l fermentor were 390 mg l-1 and 240 mg l-1, respectively, with overall productivities of 7.8 mg l -1 h-1 and 4.8 mg l-1 h-1.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-006-9023-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)