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Title 

Application of comparative genomics in developing molecular markers tightly linked to the virus resistance gene Rsv4 in soybean

 

콩 바이러스 저항성 유전자 Rsv4에 연관된 분자표지 개발에서 비교유전체학

Authors 

T Y HwangJ K MoonSeok YuKiWoung YangS MohankumarY H YuY H LeeH S KimHwan Mook KimM A S MaroofSoon Chun Jeong

Publisher 

NRC Research Press (Canadian Science Publishing)

Issue Date 

2006

Citation 

Genome, vol. 49, no. 4, pp. 380-388

Keywords 

comparative genomicslotusresistance genesoybeancomparative studygenetic linkagegenetic markergeneticsgenomicsmosaic virus

Abstract 

The Rsv4 gene confers resistance to all the known strain groups of soybean mosaic virus in soybean (Glycine max (L.) Merr.). To construct a fine genetic map near Rsv4 in soybean, we employed a comparative genomics approach that used genome sequence information of the model legume Lotus japonicus. Sequences of the soybean expressed sequence tags (ESTs) AI856415 and BF070293 mapping to one side of the Rsv4 gene showed high similarity with gene sequences of the transformation-competent artificial chromosome (TAC) clone LjT32P24 of Lotus. LjT32P24 is tightly linked to another sequenced TAC clone, LjT26I01, in Lotus. A new marker, AW307114A, developed from soybean EST AW307114, which is homologous to a Lotus gene within LjT26I01, was mapped to the other side of the Rsv4 gene. The identification of the microsyntenic relationship facilitated the development of additional 2 EST markers between BF070293-S and AW307114A bracketing the Rsv4 gene. Several other markers developed in this study were mapped to putative homoeologous or duplicated chromosomal regions in soybean. Alignment between the soybean maps indicated that Rsv4 is located near a local chromosomal rearrangement. This targeted comparative mapping serves to provide a foundation for marker-assisted selection and cloning of the Rsv4 gene.

ISSN 

0831-2796

Link 

http://dx.doi.org/10.1139/G05-111

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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