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Title 

Preparation of soluble silk peptides by food-grade proteinases

 

효소분해에 의한 가용성 실크 펩타이드의 제조

Authors 

Jae Seok HaJae Jun SongH K ChoSeung Goo Lee

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2006

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 34, no. 2, pp. 115-120

Keywords 

food-grade proteasesilk fibroinsoluble silk peptidesilk

Abstract 

Enzymatic hydrolysis of silk fibers were investigated for the preparation of soluble silk peptides by ten food-grade proteases from Bacillus, Aspergillus, and plant sources. Silk fibers were dissolved for 1 hr in a 2:1 cosolvent (50% CaCl2: ethanol) by heating at 90°C. The silk solution was filtered to remove impurity particles and desalted for 50 hours by a dialysis process to remove the used cosolvent. When the silk hydrolysis was performed at 45°C for 2 hours, most proteases from Bacillus and Aspergillus generated large amounts of insoluble aggregates. On the contrary, proteases from plant sources produced much less aggregates during prolonged incubations and also exhibited high hydrolysis activities. In regards of the solubility and broad molecular sizes of produced silk peptides, Bromelain was finally selected and applied for the enzymatic hydrolysis of silk fibers.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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