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Title 

Identification of novel genes with proapoptotic activity

Authors 

Eun Ju KangJ M KimN KimKyung Mi ParkSeong-Min ParkNam-Soon KimHyang Sook YooYoung Il YeomSoo Jung Kim

Publisher 

Korea Genome Organization

Issue Date 

2006

Citation 

Genomics & Informatics, vol. 4, no. 2, pp. 77-79

Keywords 

novel proapoptotic genesapoptosisfluorescence microscopeapoptotic nuclei

Abstract 

In order to identify novel proapoptotic genes, we screened approximately 1,000 hypothetical genes whose functions are completely unknown. After these genes were transiently expressed in HeLa cells, their nuclei images were captured using automated high-speed fluorescence microscope, through which the ratio of apoptotic nuclei was estimated. We selected genes that induce greater than 3-fold increase in apoptotic nuclei compared to that of the vector control. The candidate proapoptotic genes were sequenced and their effects on cell death were further confirmed by the additional assay, DNA fragmentation ELISA. Finally, we were able to identify 4 full-length hypothetical genes with proapoptotic activity.

ISSN 

1598-866X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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