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Title 

Oral administration of human papillomavirus type 16 E7 displayed on Lactobacillus casei induces E7-specific antitumor effects in C57/BL6 mice

 

파필로마바이러스 유산균 치료백신 개발

Authors 

Haryoung PooH M PyoT Y LeeSun-Woo YoonJ S LeeC J KimM H SungS H Lee

Publisher 

Wiley-Blackwell

Issue Date 

2006

Citation 

International Journal of Cancer, vol. 119, no. 7, pp. 1702-1709

Keywords 

antitumor effectcellular immunityHPV16 E7lactobatillus caseiPgsAvirus protein E7human papillomavirus type 16lactobacillus caseimousenonhuman

Abstract 

The mounting of a specific immune response against the human papillomavirus type 16 E7 protein (HPV16 E7) is important for eradication of HPV16 E7-expressing cancer cells from the cervical mucosa. To induce a mucosal immune response by oral delivery of the E7 antigen, we expressed the HPV16 E7 antigen on the surface of Lactobacillus casei by employing a novel display system in which the poly-γ-glutamic acid (γ-PGA) synthetase complex A (PgsA) from Bacillus subtilis (chungkookjang) was used as an anchoring motif. After surface expression of the HPV16 E7 protein was confirmed by Western blot, flow cytometry and immunofluorescence microscopy, mice were orally inoculated with L. casei-PgsA-E7. E7-specific serum IgG and mucosal IgA productions were enhanced after oral administration and significantly enhanced after boosting. Systemic and local cellular immunities were significantly increased after boosting, as shown by increased counts of lymphocytes (SI = 9.7 ± 1.8) and IFN-γ secreting cells [510 ± 86 spot-forming cells/106 cells] among splenocytes and increased IFN-γ in supernatants of vaginal lymphocytes. Furthermore, in an E7-based mouse tumor model, animals receiving orally administered L. casei-PgsA-E7 showed reduced tumor size and increased survival rate versus mice receiving control (L. casei-PgsA) immunization. These results collectively indicate that the oral administration of E7 displayed on lactobacillus induces cellular immunity and antitumor effects in mice.

ISSN 

0020-7136

Link 

http://dx.doi.org/10.1002/ijc.22035

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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