상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Pretreatment of macrophages with paclitaxel inhibits iNOS expression

Authors 

M H LiJong Soon KangHwan Mook KimY J Jeon

Issue Date 

2006

Citation 

Journal of Toxicology and Public Health, vol. 22, no. 1, pp. 103-107

Keywords 

paclitaxelmacrophagesiNOSNF-κB/Rel

Abstract 

We demonstrate that paclitaxel, an antitumor agent derived from yew tree, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Previously, paclitaxel has been known to induce iNOS gene expression in macrophages. However, in this report we described that the pretreatment of macrophages with paclitaxel (0.1 μM) for 8 h inhibited LPS-induced iNOS gene expression. Pretreatment of RAW 264.7 cells with paclitaxel significantly inhibited LPS-stimulated nitric oxide (NO) production. Western immunoblot of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression in RAW 264.7 cells. Immunocytochemical staining of iNOS further confirmed that pretreatment of macrophages with paclitaxel inhibited macrophage activation. Electrophoretic mobility shift assay showed that paclitaxel inhibited NF-κ/Rel DNA binding. Collectively, these series of experiments indicate that paclitaxel inhibits iNOS gene expression by blocking NF-κB/Rel activation.

ISSN 

1226-8399

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)