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Title 

A potential demerit of the pronuclear microinjection technique

Authors 

A G WangSun-Uk KimH B MoonByung Hwa HyunKi Hoan NamJ G SuhNam-Soon KimDae Yeul YuDong Seok Lee

Publisher 

Korean Society of Life Science

Issue Date 

2006

Citation 

Korean Journal of Life Sciences, vol. 16, no. 4, pp. 566-570

Keywords 

nuclear damagepronuclear microinjectiontransgenic mice

Abstract 

Pronuclear microinjection (PMI) is a primary method for producing transgenic mice and offers a powerful tool for investigating gene function in vivo. The method has several reported advantages and disadvantages. Here, we report another potential shortcoming. The survival rate of fertilized one cell-stage embryos was significantly reduced after PMI procedure (65.4% (1202/1838)). In addition, the proportion of embryos developing to full-term was also significantly lower than that of embryos not undergoing PMI (26.5% (319/1202) vs 41.9% (57/136)). Moreover, 3 out of 21 (14.3%) founder control mice which were non-transgene-carrying littermates of transgenic founders showed histopathological changes in their liver, which was comparable to that in of transgenic lineages (4 out of 27 (14.8%)). In conclusion, the mechanical damages in chromosomes occurring during PMI procedure may be a potential factor influencing phenotypes of transgenic mice.

ISSN 

1225-9918

Link 

http://dx.doi.org/10.5352/JLS.2006.16.4.566

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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